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mouse es cells  (Selleck Chemicals)


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    Selleck Chemicals mouse es cells
    Mouse Es Cells, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 94/100, based on 61 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+es+cells/BIX+01294/pm41781491-53-4-13
    Average 94 stars, based on 61 article reviews
    mouse es cells - by Bioz Stars, 2026-10
    94/100 stars

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    Activity Assay:

    Article Title: Uncovering the acetylation sites of Dnmt3L that regulate protein stability and differentiation potency in embryonic stem cells
    Article Snippet: To maintain naive pluripotency, mouse ES cells were maintained in 2i-LIF medium containing 3 μM CHIR99021 (BioGems) and 1 μM PD0325901 (BioGems) under the following two conditions on a 0.1% gelatin-coated tissue culture dish: FBS free or supplemented with knockout serum replacement (KSR) (Thermo Fisher Scientific). .. To inhibit G9a activity, mouse ES cells were treated with 3 μM BIX01294 (Selleckchem) for 24 h. To block de novo translation and transcription, cells were treated with 200 μg/ml cycloheximide (CHX, Sigma-Aldrich) and 7.5 μg/ml actinomycin D (Sigma-Aldrich), respectively, for the indicated number of hours. ..

    Article Title: Uncovering the acetylation sites of Dnmt3L that regulate protein stability and differentiation potency in embryonic stem cells.
    Article Snippet: To maintain naive pluripotency, mouse ES cells were maintained in 2i-LIF medium containing 3 μM CHIR99021 (BioGems) and 1 μM PD0325901 (BioGems) under the following two conditions on a 0.1% gelatin-coated tissue culture dish: FBS free or supplemented with knockout serum replacement (KSR) (Thermo Fisher Scientific). .. To inhibit G9a activity, mouse ES cells were treated with 3 μM BIX01294 (Selleckchem) for 24 h. To block de novo translation and transcription, cells were treated with 200 μg/ml cycloheximide (CHX, Sigma-Aldrich) and 7.5 μg/ml actinomycin D (Sigma-Aldrich), respectively, for the indicated number of hours. ..

    Blocking Assay:

    Article Title: Uncovering the acetylation sites of Dnmt3L that regulate protein stability and differentiation potency in embryonic stem cells
    Article Snippet: To maintain naive pluripotency, mouse ES cells were maintained in 2i-LIF medium containing 3 μM CHIR99021 (BioGems) and 1 μM PD0325901 (BioGems) under the following two conditions on a 0.1% gelatin-coated tissue culture dish: FBS free or supplemented with knockout serum replacement (KSR) (Thermo Fisher Scientific). .. To inhibit G9a activity, mouse ES cells were treated with 3 μM BIX01294 (Selleckchem) for 24 h. To block de novo translation and transcription, cells were treated with 200 μg/ml cycloheximide (CHX, Sigma-Aldrich) and 7.5 μg/ml actinomycin D (Sigma-Aldrich), respectively, for the indicated number of hours. ..

    Article Title: Uncovering the acetylation sites of Dnmt3L that regulate protein stability and differentiation potency in embryonic stem cells.
    Article Snippet: To maintain naive pluripotency, mouse ES cells were maintained in 2i-LIF medium containing 3 μM CHIR99021 (BioGems) and 1 μM PD0325901 (BioGems) under the following two conditions on a 0.1% gelatin-coated tissue culture dish: FBS free or supplemented with knockout serum replacement (KSR) (Thermo Fisher Scientific). .. To inhibit G9a activity, mouse ES cells were treated with 3 μM BIX01294 (Selleckchem) for 24 h. To block de novo translation and transcription, cells were treated with 200 μg/ml cycloheximide (CHX, Sigma-Aldrich) and 7.5 μg/ml actinomycin D (Sigma-Aldrich), respectively, for the indicated number of hours. ..



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    ( A - C ) Control and BromoTag-Mcm10 dTAG-Recql4 <t>mouse</t> ES <t>cells</t> were incubated for eight hours in the presence or absence of the PROTACs AGB1 and dTAG13, or in the presence of 2 mM hydroxyurea, before treatment for 20 minutes with EdU. Cells were analysed by high-content screening microscopy as described above for . For panels (B) and (C), EdU-negative cells (see Methods) are indicated in black. ( D ) Samples for the above high-content screening experiment were also analysed by immuno-staining with phospho-specific antibodies corresponding to Serine 4 (S4) and Serine 8 (S8) of RPA32, which is phosphorylated in response to activation of the DNA replication checkpoint pathway. ( E ) Samples from the same experiment were used to prepare cell extracts that were analysed by immunoblotting for the indicated proteins. ‘P-CHK1 (S345)’ corresponds to phosphorylation of Serine 345 of CHK1, whereas ‘P-RPA32 (S33)’ corresponds to phosphorylation of Serine 33 of RPA32, both of which are mediated by the ATR kinase in response to checkpoint activation. ( F ) Mouse ES cells of the genotype GFP-Psf1 (Control) or BromoTag-Mcm10 dTAG-Recql4 GFP-Psf1 were incubated for four hours in the presence or absence of the PROTACs AGB1 and dTAG13. <t>Live</t> cells were then stained with the DNA-binding dye SPY650-DNA (see Methods). Arrows indicate the accumulation of GFP-PSF1 on patches of constitutive heterochromatin. The scale bar corresponds to 10 µm.
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    ( A - C ) Control and BromoTag-Mcm10 dTAG-Recql4 mouse ES cells were incubated for eight hours in the presence or absence of the PROTACs AGB1 and dTAG13, or in the presence of 2 mM hydroxyurea, before treatment for 20 minutes with EdU. Cells were analysed by high-content screening microscopy as described above for . For panels (B) and (C), EdU-negative cells (see Methods) are indicated in black. ( D ) Samples for the above high-content screening experiment were also analysed by immuno-staining with phospho-specific antibodies corresponding to Serine 4 (S4) and Serine 8 (S8) of RPA32, which is phosphorylated in response to activation of the DNA replication checkpoint pathway. ( E ) Samples from the same experiment were used to prepare cell extracts that were analysed by immunoblotting for the indicated proteins. ‘P-CHK1 (S345)’ corresponds to phosphorylation of Serine 345 of CHK1, whereas ‘P-RPA32 (S33)’ corresponds to phosphorylation of Serine 33 of RPA32, both of which are mediated by the ATR kinase in response to checkpoint activation. ( F ) Mouse ES cells of the genotype GFP-Psf1 (Control) or BromoTag-Mcm10 dTAG-Recql4 GFP-Psf1 were incubated for four hours in the presence or absence of the PROTACs AGB1 and dTAG13. Live cells were then stained with the DNA-binding dye SPY650-DNA (see Methods). Arrows indicate the accumulation of GFP-PSF1 on patches of constitutive heterochromatin. The scale bar corresponds to 10 µm.

    Journal: bioRxiv

    Article Title: MCM10 and SLD-2/RECQL4 jointly activate the CMG helicase during metazoan DNA replication initiation

    doi: 10.64898/2026.04.01.715773

    Figure Lengend Snippet: ( A - C ) Control and BromoTag-Mcm10 dTAG-Recql4 mouse ES cells were incubated for eight hours in the presence or absence of the PROTACs AGB1 and dTAG13, or in the presence of 2 mM hydroxyurea, before treatment for 20 minutes with EdU. Cells were analysed by high-content screening microscopy as described above for . For panels (B) and (C), EdU-negative cells (see Methods) are indicated in black. ( D ) Samples for the above high-content screening experiment were also analysed by immuno-staining with phospho-specific antibodies corresponding to Serine 4 (S4) and Serine 8 (S8) of RPA32, which is phosphorylated in response to activation of the DNA replication checkpoint pathway. ( E ) Samples from the same experiment were used to prepare cell extracts that were analysed by immunoblotting for the indicated proteins. ‘P-CHK1 (S345)’ corresponds to phosphorylation of Serine 345 of CHK1, whereas ‘P-RPA32 (S33)’ corresponds to phosphorylation of Serine 33 of RPA32, both of which are mediated by the ATR kinase in response to checkpoint activation. ( F ) Mouse ES cells of the genotype GFP-Psf1 (Control) or BromoTag-Mcm10 dTAG-Recql4 GFP-Psf1 were incubated for four hours in the presence or absence of the PROTACs AGB1 and dTAG13. Live cells were then stained with the DNA-binding dye SPY650-DNA (see Methods). Arrows indicate the accumulation of GFP-PSF1 on patches of constitutive heterochromatin. The scale bar corresponds to 10 µm.

    Article Snippet: For the experiment in , live mouse ES cells were stained with a 4000 fold dilution of SPY650-DNA (SC501, Spirochrome), before imaging by spinning-disk confocal microscopy as described above for C. elegans .

    Techniques: Control, Incubation, High Content Screening, Microscopy, Immunostaining, Activation Assay, Western Blot, Phospho-proteomics, Staining, Binding Assay